For certain tests, 24-hour urine samples are collected at home and must be refrigerated during the collection process. Random Urine Sample 4.2. Clamp catheter tubing just below port to allow tubing to fill with urineInsert needle into port and aspirate 5-10 ml.Empty syringe contents into sterile specimen cup and send to lab. <> Processing of urine in microbiology by Rahul raj, Cytopathology Lab manual for MLT Students, Abnormalities of the Pupil copy copy copy.pptx, Westside Wellness - Mobile IV Hydration Therapy, Intensification Options after basal Insulin Revisited, health related behaviour ( ).pptx, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. Patients Preparation factors Therapeutic Drug Monitoring: different pharmacologic agents have patterns of administration, body distribution, metabolism, and elimination that affect the drug concentration as measured in the blood. Proper sample collection leads to the proper diagnosis of disease. Collect the sample in the appropriate container. The SlideShare family just got bigger. Postural effects are accentuated in patients with edema and low plasma protein values. Gauze sponges - for application on the site from which the needle is withdrawn. All samples which may contain dangerous and highly infectious viruses must be labeled HIGH RISK with a red marker pen, and handled with great care. Explain procedure, gain clients participation, Collect right amt. The availability of new laboratory techniques for unusual pathogens, makes necessary the review and update of all the steps involved in the processing of the samples. You might even have a presentation youd like to share with others. 0 Ideally microbiological specimens should be collected before beginning any treatment such as antibiotics or using antiseptics. You might even have a presentation youd like to share with others. n&)AE(y CtQuIx`_9BP&%o-)}pJ6 sd.%E>KPL\,P)4v09F.r#m".z?/G h:"dgmzQeO[zq2y]Q#Fp6:l2o;)FWPY(b;9OI+={pG~x2% nH6Ixj?bt*Gx||$w0eRT@(YlvHGdUiB0 Knw9+ h There are some medications and other substances that can alter the results of the analyses. Specimen labelling and processing: Activate your 30 day free trialto continue reading. Role of specimen collection in infectious disease by Dr. T. V. Rao MD . specimen collection, preservation and transportation ppt. What containers are required to collect the specimens? microbiology. Impact of Incorrect Volume on PT and APTT The APTT is the most sensitive to variations in final citrate concentration. E E Urine specimen stability under shipping conditions other than those recommended has not been evaluated. Global Specimen Containers Market Outlook 2021. Urine collection. Label the collection tubes at the bedside or drawing area. 1 0 obj test, Urine Collection Technician: Proficiency Demonstrations - Urine specimen collectors. It's FREE! 282-288, 297-298, 360-361 and 380-381 - . Collecting the urine specimen is awkward but not in itself uncomfortable (An infection, however, can create a burning sensation during urination.). Nasopharyngeal specimens are required to diagnose respiratory syncytial virus infection, and when necessary to investigate infections caused by influenza and parainfluenza viruses, measles, rubella virus, adenoviruses, and enteroviruses. Factor s Assay) There are certain tests performed after administration of drugs / substances such as: glucose tolerance tests (GTT) performed after glucose administration or dynamic tests (of stimulation or inhibition) for different hormones ( e.g. Job Description: Job Summary: A Clinical Manager is responsible for delivery of effective laboratory service by providing an environment that supports the productivity, competency and morale of technical and support staff. Expired tubes with low vacuum (rare). Refrigerated immediately at 4C. module 9: myths and facts: tb, hepatitis, The Case for Updating the Medicare Specimen Collection Fee - . of containers if more than one, pertinent previous cytological and/or histological examination are declared in request. Collection of Epidemiologic Data. 5 0 obj It has millions of presentations already uploaded and available with 1,000s more being uploaded by its users every day. For body fluid collection provide the patient with the suitable container and collection instruction . We've encountered a problem, please try again. Collect the sample within 5 days of the onset of the symptoms, and the second sample 5-10 days later. Perform the venipuncture. Heparin (Anti-Coagulant) Used to collect whole blood or plasma Binds to anti-thrombin III to inhibit Xa, IXa, and thrombin Nominal concentration of 12 30 U/mL Heparin binds calcium so ionized calcium must be collected using Calcium Titrated or Electrolyte Balanced heparin, Citrate (Anti-Coagulant) Citrate is used for collection of coagulation tests Acts by binding calcium Nominal concentration of 3.2% (0.109 mol/L) Recent move to 3.2% from 3.8% to get more consistent results for Prothrombin Time, particularly for more sensitive reagents Tubes must be properly filled to within +/- 10% of assigned collection volume, Clot Activator Shortens Time to Clot In vitro activation of clotting system to enhance clot formation A silica clot activator is attached to the tube wall with a silicone surfactant Requires mixing for optimal affect Time to clot is 15-30 minutes instead of 1 hour, Serum Separator Gel Separates Serum from Cells Polymer gel with specific gravity between that of serum and cells Migrates and forms a barrier during centrifugation Separation of cells from serum stops metabolic and hemolytic effects Certain analytes and therapeutic drugs may bind to gel over time, Labelling the Sample A properly labelled sample is essential so that the results of the test match the patient. Appropriate samples from the appropriate site and an adequate amount should be collected. ; worn to protect the patient and the phlebotomist. Collect skin scrapings in a dry sterile container. It's FREE! Name and address of the hospital or health centre. Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. Free access to premium services like Tuneln, Mubi and more. Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. Holder/Adapter - use with the evacuated collection system. The phlebotomist must correctly identify the patient before specimen collection. z.$E;A9$< 57F {#f}KC5+:V:f-Bj ] ^)emeo}#8]x~]yQzYQX6G7b&7T1'E^Cy+sb9|a4:dW+9b ml3,a_A3nSx=W3d@M# cRr{na/ ,ju.Zje^-F38x% &c&^o3XhG(b3xz?I%~c5/-\^q%,n._| Y+ Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. Collect 5-10 ml of venous blood in a dry sterile, screw-cup glass tube or bottle. Effects of Exercise: Muscular activity has both transient and longer lasting effects. Regulations for transporting samples come from . Some sex hormones need special timing as regard cycle : (1)FSH,LH (3rd day of cycal {follicular phase}) (2)Progesterone (21 day of cycle{ luteal phase}) Other Factors: Age, gender, and pregnancy have an influence on laboratory testing. nurses often assume the responsibility of specimen collection. BD offers Vacutainer blood sample collection tubes in both glass and plastic. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. Many blood-based analyses require collection into yellow-topped acid-citrate-dextrose tubes and overnight shipment of the chilled tubes to the laboratory. collecting liquid specimens in a needle and syringe 10 General guidelines Prompt processing is important The quicker the specimen is processed the better Pathogens may die in transit thus leading to false negative results (ideally specimens for culture should arrive in the lab within 30 minutes of collection) Many drugs will have "peak" and "trough" levels that vary according to dosage levels and intervals. You can read the details below. By accepting, you agree to the updated privacy policy. PowerShow.com is a leading presentation sharing website. sample collection. Patients Preparation factors ,cont. PowerPoint presentation 'Specimen Collection and Transport' is the property of its rightful owner. Follow patient instructions prior to specimen collection Including the proper order of blood draw when multiple tubes are required. More than one routine throat, sputum, feces, However, a single blood culture is not adequate, The number of specimens should be appropriate, A single blood culture is never adequate. <> Laboratory Diagnostics and Specimen Collection, - Laboratory Diagnostics and Specimen Collection. PowerShow.com is brought to you byCrystalGraphics, the award-winning developer and market-leading publisher of rich-media enhancement products for presentations. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. xK@sLngf?`'MqXC~//0p: Fp1{!BDb2F)8^?Z(qDP~#:B^}tGzMAP|fzmF"ne`R_D>$"e_v`H1^Vge[ZH@zg$f =)Cu='o"pq.:89]mzM:YRl4OMy}y9(k{V5hm[!rw}A8q/g;`T],p*#Y\&}?il&8r;NF|D A single sample is required for investigating newborn infants with congenital defects, determining immunity to rubella or other viruses. An exception to this rule, throat swabs for S. Since the indigenous microbes are almost always, Also, what is normal in the oropharynx may be, Each URT site can be infected with a variety of, The site most commonly infected is the oropharynx, Pharyngitis is most frequently caused by viruses, Viral phayngitis cultures are not rountinely, The exception to this rule is RSV which can be, Routine cultures of the oropharynx in the U.S.A, S. pyogenes pharyngitis is frequently associated, S. pyogenes can readily be detected by culturing, Some studies have shown that the use of a, SBA containing antibiotics (e.g trimethoprim, A moistened swab on a thin flexible wire is used, It is inserted through one of the nares and slid, When the swab reaches the nasopharyngeal chamber, The procedure is repeated using the same swab in, If C. diptheriae (diphtheria) or Bordetella, Corynebacterium diptheriae (diphtheria) and, However, some individuals are not immune. Well convert it to an HTML5 slideshow that includes all the media types youve already added: audio, video, music, pictures, animations and transition effects. hbbd```b``"j@$SX&/O`r?VDtE$ydK000R k3 for a Condition To detect subclinical disease (screening) For Research Purposes, Proper procedures must be followed to assure the integrity of the specimen and its safe and expedient delivery to the laboratory as well as its processing once in the laboratory It is the job of the phlebotomist to make sure that all the guidelines of the Laboratories are met and implemented in order to deliver a suitable sample for analysis so that an accurate and precise result will be issued, Several essential steps are required for every successful collection procedure. 363 0 obj <> endobj The type of specimen, the appropriate time to obtain the sample, the way of sampling, the storage and transport are critical points in the diagnostic process. Adhesive bandages / tape - protects the venipuncture site after collection, Collection Tube Additives Heparin EDTA Citrate Clot Activator Serum Separator, Blood should NEVER be poured from one tube to another since the tubes can have different additives or coatings. The Phlebotomist/Courier practices safe driving . Once collected samples are recommended to emulsify or mixed properly with viral transport medium (VTM). Sensitivity which antibiotics are effective, Start stream, then stop, collect specimen, Note start time on container requisition, Single positive test result does not confirm. Tap here to review the details. 2 0 obj Place about 4-8 g of feces (small spoon) in a clean, dry, leak-proof container. Perform the venipuncture. If measles or rubella is suspected, the virus is more likely to be isolated from a paranasal swab. 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